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Image Search Results
Journal: bioRxiv
Article Title: LiverZap: A chemoptogenetic tool for global and locally restricted hepatocyte ablation to study cellular behaviours in liver regeneration
doi: 10.1101/2023.03.13.531762
Figure Lengend Snippet: A-I tg(tp1:H2B-mCherry) -positive nuclei (magenta) serve as proxy for biliary network remodelling during ablation and regeneration. 10 µm masked maximum intensity projections are shown. J Distribution of the nearest neighbour distances displayed by mCherry high nuclei populations in controls and following LiverZap ablation (N=2, n>3); C=Control, M=Mild, S=Severe. K Schematic showing sequence for live imaging experiment. L-S Selected frames from time-lapse imaging (VideoS2) of tg(tp1:eGFP)- expressing BECs (green) in control (L-M) and LiverZap-ablated livers (N-S). BECs exhibit dynamic cellular behaviours including cell aggregation (N-O), loss (P-Q) and gain of new cell-cell contacts (R-S) during the phase hepatocytes die. Magenta asterisks mark the same cell across the entire timelapse. T-V’ E-cadherin expression (cyan) in control livers (T-T’), distant ROI (U-U’) and ablated ROI (V-V’). tg(tp1:H2B-mCherry) visualizes BECs (magenta). 10 µm maximum intensity projections shown. Yellow arrowheads highlight selected BECs.
Article Snippet: Antibodies used included: mouse α-2F11 (1:1000; gift from Julian Lewis), goat α-Hnf4a (1:100; Santa Cruz, cat# sc-6556, lot# B1605),
Techniques: Sequencing, Imaging, Expressing